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lb 005 human clusterin protein r d systems  (R&D Systems)


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    R&D Systems lb 005 human clusterin protein r d systems
    Lb 005 Human Clusterin Protein R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lb+005+human+clusterin+protein+r+d+systems/Recombinant+Human+IL-1+beta%2FIL-1F2+Protein/pm37494190-256-27-31
    Average 97 stars, based on 1115 article reviews
    lb 005 human clusterin protein r d systems - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    cDNA Synthesis:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    SYBR Green Assay:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    Chromatin Immunoprecipitation:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    Reporter Assay:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    Mass Spectrometry:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    Binding Assay:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    RNA Sequencing:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..

    Software:

    Article Title: Astrocytic response mediated by the CLU risk allele inhibits OPC proliferation and myelination in a human iPSC model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-GFAP DAKO Cat# N1506; RRID: AB_10013482 Mouse monoclonal anti-S100b Sigma-Aldrich Cat# S2532; RRID: AB_477499 Rabbit polyclonal anti-OLIG2 EMD Millipore Cat# AB9610; RRID: AB_570666 Mouse monoclonal IgM anti-O4 Sigma-Aldrich Cat# O7139; RRID: AB_477662 Anti-O4 MicroBeads Miltenyi Biotec Cat# 130-096-670; RRID: AB_2847907 Goat polyclonal anti-SOX10 R&D systems Cat# AF2864; RRID: AB_442208 Rat monoclonal anti-MBP Millipore Cat# MAB386; RRID: AB_94975 Rabbit polyclonal anti-Cleaved Caspase-3 Cell Signaling Technology Cat# 9661; RRID: AB_2341188 Rabbit polyclonal anti-STAT1 Santa Cruz Cat# sc-346; RRID: AB_632435 Rabbit monoclonal anti-Phospho-STAT1 Cell Signaling Technology Cat# 9167; RRID: AB_561284 Rabbit monoclonal anti-CLUSTERIN Cell Signaling Technology Cat# 34642; RRID: AB_2799057 Mouse monoclonal anti-CLUSTERIN Santa Cruz Cat# sc-5289; RRID: AB_673566 Mouse monoclonal anti-CXCL10 R&D systems Cat# MAB266; RRID: AB_2261309 Rabbit polyclonal anti-TDP-43 Proteintech Cat# 10782-2-AP; RRID: AB_615042 Goat polyclonal anti- Galectin-7 R&D systems Cat# AF1339; RRID: AB_2297076 Mouse monoclonal anti-hnRNP E2 Santa Cruz Cat# sc-101136; RRID: AB_1124684 Rabbit polyclonal anti- Neurofilament 200 Sigma-Aldrich Cat# N4142; RRID: AB_477272 Goat polyclonal anti-SOX9 R&D systems Cat# AF3075; RRID: AB_2194160 Biological samples Human brain tissue Banner Sun Health Research Institute www.brainandbodydonationprogram.org Chemicals, peptides, and recombinant proteins DMEM/F12 GIBCO Cat# 11330-032 Matrigel Corning Cat# CB40230 mTeSR1 Stem Cell Technologies Cat# 85850 Y-27632 Reprocell Cat# 04-0012-10 Accutase Sigma-Aldrich Cat# A6964 N2 Life Technologies Cat# 17502048 B27 Life Technologies Cat# 12587010 GlutaMax GIBCO Cat# 35050079 NEAA Thermo Fisher Scientific Cat# 11140076 CHIR99021 Cellagen Technology Cat# C2477-50 SB431542 Stemgent Cat# 04-0010 LDN-193189 Stemgent Cat# 04-0074 Smoothened agonist (SAG) EMD Millipore Cat# 566660 bFGF PeproTech Cat# 100-18B EGF PeproTech Cat# 100-15 Retinoic acid Sigma-Aldrich Cat# R2625 CNTF R&D systems Cat# 257-NT-050 PDGFAA R&D systems Cat# 221-AA-050 IGF-1 R&D systems Cat# 291-G1-200 HGF R&D systems Cat# 294-HG-025 NT3 EMD Millipore Cat# GF031 3,30,5-Triiodo-L-thyronine (T3) Sigma-Aldrich Cat# T2877 Biotin Sigma-Aldrich Cat# 4639 (Continued on next page) 20 Cell Reports 42, 112841, August 29, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Dibutyryl-cAMP Sigma-Aldrich Cat# D0627 L-Ascorbic acid Sigma-Aldrich Cat# A4403 Inuslin Sigma-Aldrich Cat# I9278 TGFa Stemcell Techology Cat# 78157.1 IL1b R&D systems Cat# 201-LB-005 Human Clusterin Protein R&D Systems Cat# 2937-HS-050 Critical commercial assays Tetro cDNA Synthesis kit BioLINE Cat# Bio-65043 SYBR Green Master Mix Thermo Scientific Cat# F416L P3 4D nucleofection kit Lonza Cat# V4XP-3024 Human Clusterin Quantikine ELISA Kit R&D Systems Cat# DCLU00 HUMAN IP-10/CXCL10 ELISA KIT MilliporeSigma Cat# RAB0119 Click-iTTM Plus EdU Cell Proliferation Kit Invitrogen Cat# C10637 Magna ChIPTM A/G Chromatin Immunoprecipitation Kit Sigma Cat# 17-10085 CUT&RUN Assay Kit Cell Signaling Technology Cat# 86652 Dual-Luciferase Reporter Assay System Promega Cat# E1910 Alexa FluorTM 488 Tyramide SuperBoostTM Kit, goat anti-mouse IgG Invitrogen Cat# B40912 hPSC Genetic Analysis Kit Stemcell Techology Cat# 07550 PureLinkTM Genomic DNA Mini Kit Invitrogen Cat# K182001 Deposited data Mass spectrometry analysis of differential binding to the C vs. T allele by nuclear proteins This paper MassIVE: MSV000088660 RNA-seq analysis of C/C vs. T/T or C/T astrocytes This paper GEO: GSE193218 Experimental models: Cell lines ADRC18 fibroblast UCI-ADRC N/A AG14048 fibroblasts Coriell Cat# AG14048 AG06869 fibroblasts Coriell Cat# AG06869 Human primary astrocytes ScienCell Cat# 1800 Oligonucleotides See Tables S6 and S7 for details N/A N/A Software and algorithms NIS-Elements AR Nikon RRID: SCR_014329 ZEN 3.1 Carl Zeiss RRID:SCR_013672 Image Lab Software Bio-rad RRID:SCR_014210 Fiji (ImageJ) Fiji (ImageJ) RRID: SCR_002285 Graphpad Prism 9 Graphpad Software RRID: SCR_002798 Biorender Biorender RRID:SCR_018361 ..



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